Sequential fractionation of feruloylated hemicelluloses and oligosaccharides from wheat bran using subcritical water and xylanolytic enzymes

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Sequential fractionation of feruloylated hemicelluloses and oligosaccharides from wheat bran using subcritical water and xylanolytic enzymes†

Wheat bran is a major by-product of cereal production that still has limited use for advanced nutritional and material applications. A sequential process using subcritical water, membrane filtration and selective enzymatic treatments has been designed for the combined fractionation of functional high molar mass hemicelluloses (over 10 g mol) and oligosaccharides from wheat bran. This process no...

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Inhibition of Intestinal α-Glucosidase and Glucose Absorption by Feruloylated Arabinoxylan Mono- and Oligosaccharides from Corn Bran and Wheat Aleurone

The effect of feruloylated arabinoxylan mono- and oligosaccharides (FAXmo) on mammalian α-glucosidase and glucose transporters was investigated using human Caco-2 cells, rat intestinal acetone powder, and Xenopus laevis oocytes. The isolated FAXmo from wheat aleurone and corn bran were identified to have degree of polymerization (DP) of 4 and 1, respectively, by HPLC-MS. Both FAXmo extracts wer...

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Isolation and identification of feruloylated arabinoxylan mono- and oligosaccharides from undigested and digested maize and wheat

Feruloylated arabinoxylan mono- and oligosaccharides (F-AXOS) are a subject of interest because of their prebiotic and antioxidant properties. We aimed at isolating and identifying F-AXOS from maize, wheat, wheat bran and wheat aleurone using HPLC and LC-MS/MS. Prior to extraction of F-AXOS, samples were subjected to either simulated gastric fluid with enzymes (gastric) or without enzymes (pH) ...

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Rice bran feruloylated oligosaccharides activate dendritic cells via Toll-like receptor 2 and 4 signaling.

This work presents the effects of feruloylated oligosaccharides (FOs) of rice bran on murine bone marrow-derived dendritic cells (BMDCs) and the potential pathway through which the effects are mediated. We found that FOs induced phenotypic maturation of DCs, as shown by the increased expression of CD40, CD80/CD86 and MHC-I/II molecules. FOs efficiently induced maturation of DCs generated from C...

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ژورنال

عنوان ژورنال: Green Chemistry

سال: 2017

ISSN: 1463-9262,1463-9270

DOI: 10.1039/c6gc03473j